Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification.
Identifieur interne : 000346 ( Main/Exploration ); précédent : 000345; suivant : 000347Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification.
Auteurs : Zhao Wang [Suède] ; Prashant Mohan-Anupama Pawar [Suède] ; Marta Derba-Maceluch [Suède] ; Mattias Hedenström [Suède] ; Sun-Li Chong [Finlande] ; Maija Tenkanen [Finlande] ; Leif J. Jönsson [Suède] ; Ewa J. Mellerowicz [Suède]Source :
- Frontiers in plant science [ 1664-462X ] ; 2020.
Abstract
Fast-growing broad-leaf tree species can serve as feedstocks for production of bio-based chemicals and fuels through biochemical conversion of wood to monosaccharides. This conversion is hampered by the xylan acetylation pattern. To reduce xylan acetylation in the wood, the Hypocrea jecorina acetyl xylan esterase (HjAXE) from carbohydrate esterase (CE) family 5 was expressed in hybrid aspen under the control of the wood-specific PtGT43B promoter and targeted to the secretory pathway. The enzyme was predicted to deacetylate polymeric xylan in the vicinity of cellulose due to the presence of a cellulose-binding module. Cell-wall-bound protein fractions from developing wood of transgenic plants were capable of releasing acetyl from finely ground wood powder, indicative of active AXE present in cell walls of these plants, whereas no such activity was detected in wild-type plants. The transgenic lines grew in height and diameter as well as wild-type trees, whereas their internodes were slightly shorter, indicating higher leaf production. The average acetyl content in the wood of these lines was reduced by 13%, mainly due to reductions in di-acetylated xylose units, and in C-2 and C-3 mono-acetylated xylose units. Analysis of soluble cell wall polysaccharides revealed a 4% reduction in the fraction of xylose units and an 18% increase in the fraction of glucose units, whereas the contents of cellulose and lignin were not affected. Enzymatic saccharification of wood from transgenic plants resulted in 27% higher glucose yield than for wild-type plants. Brunauer-Emmett-Teller (BET) analysis and Simons' staining pointed toward larger surface area and improved cellulose accessibility for wood from transgenic plants compared to wood from wild-type plants, which could be achieved by HjAXE deacetylating xylan bound to cellulose. The results show that CE5 family can serve as a source of enzymes for in planta reduction of recalcitrance to saccharification.
DOI: 10.3389/fpls.2020.00380
PubMed: 32322259
PubMed Central: PMC7156598
Affiliations:
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Mellerowicz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32322259</idno><idno type="pmid">32322259</idno><idno type="doi">10.3389/fpls.2020.00380</idno><idno type="pmc">PMC7156598</idno><idno type="wicri:Area/Main/Corpus">000337</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000337</idno><idno type="wicri:Area/Main/Curation">000337</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000337</idno><idno type="wicri:Area/Main/Exploration">000337</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification.</title><author><name sortKey="Wang, Zhao" sort="Wang, Zhao" uniqKey="Wang Z" first="Zhao" last="Wang">Zhao Wang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Pawar, Prashant Mohan Anupama" sort="Pawar, Prashant Mohan Anupama" uniqKey="Pawar P" first="Prashant Mohan-Anupama" last="Pawar">Prashant Mohan-Anupama Pawar</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Derba Maceluch, Marta" sort="Derba Maceluch, Marta" uniqKey="Derba Maceluch M" first="Marta" last="Derba-Maceluch">Marta Derba-Maceluch</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Hedenstrom, Mattias" sort="Hedenstrom, Mattias" uniqKey="Hedenstrom M" first="Mattias" last="Hedenström">Mattias Hedenström</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Chong, Sun Li" sort="Chong, Sun Li" uniqKey="Chong S" first="Sun-Li" last="Chong">Sun-Li Chong</name><affiliation wicri:level="4"><nlm:affiliation>Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Food and Environmental Sciences, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Tenkanen, Maija" sort="Tenkanen, Maija" uniqKey="Tenkanen M" first="Maija" last="Tenkanen">Maija Tenkanen</name><affiliation wicri:level="4"><nlm:affiliation>Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.</nlm:affiliation><country xml:lang="fr">Finlande</country><wicri:regionArea>Department of Food and Environmental Sciences, University of Helsinki, Helsinki</wicri:regionArea><orgName type="university">Université d'Helsinki</orgName><placeName><settlement type="city">Helsinki</settlement><region type="région" nuts="2">Uusimaa</region></placeName></affiliation></author><author><name sortKey="Jonsson, Leif J" sort="Jonsson, Leif J" uniqKey="Jonsson L" first="Leif J" last="Jönsson">Leif J. Jönsson</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. Mellerowicz</name><affiliation wicri:level="1"><nlm:affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå</wicri:regionArea><wicri:noRegion>Umeå</wicri:noRegion></affiliation></author></analytic><series><title level="j">Frontiers in plant science</title><idno type="ISSN">1664-462X</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Fast-growing broad-leaf tree species can serve as feedstocks for production of bio-based chemicals and fuels through biochemical conversion of wood to monosaccharides. This conversion is hampered by the xylan acetylation pattern. To reduce xylan acetylation in the wood, the <i>Hypocrea jecorina</i> acetyl xylan esterase (<i>Hj</i>AXE) from carbohydrate esterase (CE) family 5 was expressed in hybrid aspen under the control of the wood-specific <i>Pt</i>GT43B promoter and targeted to the secretory pathway. The enzyme was predicted to deacetylate polymeric xylan in the vicinity of cellulose due to the presence of a cellulose-binding module. Cell-wall-bound protein fractions from developing wood of transgenic plants were capable of releasing acetyl from finely ground wood powder, indicative of active AXE present in cell walls of these plants, whereas no such activity was detected in wild-type plants. The transgenic lines grew in height and diameter as well as wild-type trees, whereas their internodes were slightly shorter, indicating higher leaf production. The average acetyl content in the wood of these lines was reduced by 13%, mainly due to reductions in di-acetylated xylose units, and in C-2 and C-3 mono-acetylated xylose units. Analysis of soluble cell wall polysaccharides revealed a 4% reduction in the fraction of xylose units and an 18% increase in the fraction of glucose units, whereas the contents of cellulose and lignin were not affected. Enzymatic saccharification of wood from transgenic plants resulted in 27% higher glucose yield than for wild-type plants. Brunauer-Emmett-Teller (BET) analysis and Simons' staining pointed toward larger surface area and improved cellulose accessibility for wood from transgenic plants compared to wood from wild-type plants, which could be achieved by <i>Hj</i>AXE deacetylating xylan bound to cellulose. The results show that CE5 family can serve as a source of enzymes for <i>in planta</i> reduction of recalcitrance to saccharification.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32322259</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-462X</ISSN><JournalIssue CitedMedium="Print"><Volume>11</Volume><PubDate><Year>2020</Year></PubDate></JournalIssue><Title>Frontiers in plant science</Title><ISOAbbreviation>Front Plant Sci</ISOAbbreviation></Journal><ArticleTitle>Hybrid Aspen Expressing a Carbohydrate Esterase Family 5 Acetyl Xylan Esterase Under Control of a Wood-Specific Promoter Shows Improved Saccharification.</ArticleTitle><Pagination><MedlinePgn>380</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fpls.2020.00380</ELocationID><Abstract><AbstractText>Fast-growing broad-leaf tree species can serve as feedstocks for production of bio-based chemicals and fuels through biochemical conversion of wood to monosaccharides. This conversion is hampered by the xylan acetylation pattern. To reduce xylan acetylation in the wood, the <i>Hypocrea jecorina</i> acetyl xylan esterase (<i>Hj</i>AXE) from carbohydrate esterase (CE) family 5 was expressed in hybrid aspen under the control of the wood-specific <i>Pt</i>GT43B promoter and targeted to the secretory pathway. The enzyme was predicted to deacetylate polymeric xylan in the vicinity of cellulose due to the presence of a cellulose-binding module. Cell-wall-bound protein fractions from developing wood of transgenic plants were capable of releasing acetyl from finely ground wood powder, indicative of active AXE present in cell walls of these plants, whereas no such activity was detected in wild-type plants. The transgenic lines grew in height and diameter as well as wild-type trees, whereas their internodes were slightly shorter, indicating higher leaf production. The average acetyl content in the wood of these lines was reduced by 13%, mainly due to reductions in di-acetylated xylose units, and in C-2 and C-3 mono-acetylated xylose units. Analysis of soluble cell wall polysaccharides revealed a 4% reduction in the fraction of xylose units and an 18% increase in the fraction of glucose units, whereas the contents of cellulose and lignin were not affected. Enzymatic saccharification of wood from transgenic plants resulted in 27% higher glucose yield than for wild-type plants. Brunauer-Emmett-Teller (BET) analysis and Simons' staining pointed toward larger surface area and improved cellulose accessibility for wood from transgenic plants compared to wood from wild-type plants, which could be achieved by <i>Hj</i>AXE deacetylating xylan bound to cellulose. The results show that CE5 family can serve as a source of enzymes for <i>in planta</i> reduction of recalcitrance to saccharification.</AbstractText><CopyrightInformation>Copyright © 2020 Wang, Pawar, Derba-Maceluch, Hedenström, Chong, Tenkanen, Jönsson and Mellerowicz.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Zhao</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pawar</LastName><ForeName>Prashant Mohan-Anupama</ForeName><Initials>PM</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Derba-Maceluch</LastName><ForeName>Marta</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hedenström</LastName><ForeName>Mattias</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chong</LastName><ForeName>Sun-Li</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tenkanen</LastName><ForeName>Maija</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Department of Food and Environmental Sciences, University of Helsinki, Helsinki, Finland.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jönsson</LastName><ForeName>Leif J</ForeName><Initials>LJ</Initials><AffiliationInfo><Affiliation>Department of Chemistry, KBC Chemical-Biological Centre, Umeå University, Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mellerowicz</LastName><ForeName>Ewa J</ForeName><Initials>EJ</Initials><AffiliationInfo><Affiliation>Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, Umeå, Sweden.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>04</Month><Day>08</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Plant Sci</MedlineTA><NlmUniqueID>101568200</NlmUniqueID><ISSNLinking>1664-462X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Populus</Keyword><Keyword MajorTopicYN="N">acetyl</Keyword><Keyword MajorTopicYN="N">acetyl xylan esterase</Keyword><Keyword MajorTopicYN="N">enzymatic saccharification</Keyword><Keyword MajorTopicYN="N">hybrid aspen</Keyword><Keyword MajorTopicYN="N">xylan</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>12</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>03</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>4</Month><Day>24</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">24450583</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Finlande</li><li>Suède</li></country><region><li>Uusimaa</li></region><settlement><li>Helsinki</li></settlement><orgName><li>Université d'Helsinki</li></orgName></list><tree><country name="Suède"><noRegion><name sortKey="Wang, Zhao" sort="Wang, Zhao" uniqKey="Wang Z" first="Zhao" last="Wang">Zhao Wang</name></noRegion><name sortKey="Derba Maceluch, Marta" sort="Derba Maceluch, Marta" uniqKey="Derba Maceluch M" first="Marta" last="Derba-Maceluch">Marta Derba-Maceluch</name><name sortKey="Hedenstrom, Mattias" sort="Hedenstrom, Mattias" uniqKey="Hedenstrom M" first="Mattias" last="Hedenström">Mattias Hedenström</name><name sortKey="Jonsson, Leif J" sort="Jonsson, Leif J" uniqKey="Jonsson L" first="Leif J" last="Jönsson">Leif J. Jönsson</name><name sortKey="Mellerowicz, Ewa J" sort="Mellerowicz, Ewa J" uniqKey="Mellerowicz E" first="Ewa J" last="Mellerowicz">Ewa J. Mellerowicz</name><name sortKey="Pawar, Prashant Mohan Anupama" sort="Pawar, Prashant Mohan Anupama" uniqKey="Pawar P" first="Prashant Mohan-Anupama" last="Pawar">Prashant Mohan-Anupama Pawar</name></country><country name="Finlande"><region name="Uusimaa"><name sortKey="Chong, Sun Li" sort="Chong, Sun Li" uniqKey="Chong S" first="Sun-Li" last="Chong">Sun-Li Chong</name></region><name sortKey="Tenkanen, Maija" sort="Tenkanen, Maija" uniqKey="Tenkanen M" first="Maija" last="Tenkanen">Maija Tenkanen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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